Evaluation of the specific intermolecular interactions responsible for chiral selectivity for phenylalanine analogs in subcritical fluid chromatography

A set of phenyl ring-substituted N-t-butoxycarbonyl-phenylalanine analogs w ere chirally resolved using an alpha-Burke 2 Pirkle-type chiral column unde r subcritical fluid conditions. Various mobile phase modifiers were used to elute the chiral analytes, resulting in different selectivity factors for each analog. The observed selectivity factors were accurately modeled based on the bulk solvation parameters for each mobile phase modifier. The resul ting model equation was used to predict the selectivity factors using an ad ditional modifier not included in the model building data set. The predicti ve ability of the model was demonstrated to be quite good for this limited range of analogs and mobile phase modifiers, (C) 1999 Wiley-Liss, Inc.