I. Fitos et al., Stereoselective allosteric binding interaction on human serum albumin between ibuprofen and lorazepam acetate, CHIRALITY, 11(2), 1999, pp. 115-120
The effect of ibuprofen enantiomers on the stereoselective binding of 3-acy
loxy-1,4-benzodiazepines to human serum albumin (HSA) was studied using bot
h native and Sepharose-immobilized protein. (S)-Lorazepam acetate exhibited
considerably enhanced binding, especially in the presence of (+)-(S)-ibupr
ofen. The phenomenon is an indication of cooperative allosteric interaction
between different binding sites during multiple cobinding of two ligands.
(C) 1999 Wiley-Liss, Inc.