Polyfunctional chemically bonded stationary phase for reversed phase high-performance liquid chromatography

Preparation of a chemically-bonded-silica stationary phase (SG-MIX), with d ifferent polar and nonpolar functional groups such as hydroxyl (-OH), amino (-NH2), cyano (-CN), phenyl (-Ph), octyl (-C-8) and octadecyl (-C-18), is described. The characteristics of the prepared packing was done by physicoc hemical methods such as elemental analysis, FTIR spectroscopy, solid-state nuclear magnetic resonance (NMR), chromatography (GC and HPLC) as well as b y analysis of quantitative structure-retention relationships (QSRR). The po lyfunctional SC-MIX phase possesses properties of a reversed-phase material modified by specific inputs to retention due to interactions of analytes w ith polar functionalities on the surface of adsorbent. The new type of pack ing was successfully used for both analytical and preparative separation of finasteride from its metabolite, N-methylfinasteride.