Jj. Pesek et al., Synthesis and characterization of long chain alkyl stationary phases on a silica hydride surface, CHROMATOGR, 48(9-10), 1998, pp. 631-636
C-22 and C-30 alkyl chains were attached to a silica hydride surface by a h
ydrosilation reaction. In each case both moieties were successfully bonded
by either metal complex catalysis or free radical initiation. Carbon analys
is, DRIFT spectra and C-13 CP-MAS NMR spectra confirm the bonding of the ol
efin to silica hydride. Conditions for maximum coverage were determined by
a simplex procedure. Chromatographic evaluation of these long chain bonded
phases was accomplished by separations of several types of mixtures: alkyl
substituted benzenes, polycyclic aromatic hydrocarbons; carotenoids, tocoph
erols; tricyclic antidepressants; and peptides. The C-30 column proved sign
ificantly better for separating the carotenoid and antidepressant mixtures
and somewhat better for the peptide sample.