Determination of heterocyclic aromatic amines in fried beefsteak, meat extract, and fish by capillary zone electrophoresis

Citation
C. Mardones et al., Determination of heterocyclic aromatic amines in fried beefsteak, meat extract, and fish by capillary zone electrophoresis, CHROMATOGR, 48(9-10), 1998, pp. 700-706
Citations number
17
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
CHROMATOGRAPHIA
ISSN journal
00095893 → ACNP
Volume
48
Issue
9-10
Year of publication
1998
Pages
700 - 706
Database
ISI
SICI code
0009-5893(199811)48:9-10<700:DOHAAI>2.0.ZU;2-V
Abstract
A capillary zone electrophoretic (CZE) method has been developed and optimi zed for the simultaneous determination of 2-amino-3,8-dimethylimidazo[4,5-f ]quinoline (IQ), 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), 2-a mino-3,4,8-trimethylimidazo[4,5-f] quin-oxaline (4,8-DiMeIQx), and 2-amino- 1-methyl-6-phenylimidazo[4,5-b]pyridine (PHIP) in fried beefsteak, meat ext ract, and baked salmon. Solid-phase extraction (the Gross methodology) was tested for isolating the amines. Under optimum capillary electrophoresis co nditions the proposed method separated heterocyclic aromatic amines (HAAs) within 20 min. Diode-array UV photometric detection was used and peak ident ities were confirmed by comparison of spectra. The limits of detection of t he method were between 0.05 and 0.22 mu g mL(-1) with hydrodynamic injectio n, and from 0.08 to 0.16 mu g mL(-)1 with electrokinetic injection; injecti on reproducibility (RSD %) was better with pressure injection (4.7-7.9 %) t han with electrokinetic injection (8.6-20.7 %). The method was applied to t he analysis of real and spiked samples, recoveries ranged from 6 % for PHIP to 91 % for 4,8-DiMeIQx from various cooked foods.