C. Mardones et al., Determination of heterocyclic aromatic amines in fried beefsteak, meat extract, and fish by capillary zone electrophoresis, CHROMATOGR, 48(9-10), 1998, pp. 700-706
A capillary zone electrophoretic (CZE) method has been developed and optimi
zed for the simultaneous determination of 2-amino-3,8-dimethylimidazo[4,5-f
]quinoline (IQ), 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), 2-a
mino-3,4,8-trimethylimidazo[4,5-f] quin-oxaline (4,8-DiMeIQx), and 2-amino-
1-methyl-6-phenylimidazo[4,5-b]pyridine (PHIP) in fried beefsteak, meat ext
ract, and baked salmon. Solid-phase extraction (the Gross methodology) was
tested for isolating the amines. Under optimum capillary electrophoresis co
nditions the proposed method separated heterocyclic aromatic amines (HAAs)
within 20 min. Diode-array UV photometric detection was used and peak ident
ities were confirmed by comparison of spectra. The limits of detection of t
he method were between 0.05 and 0.22 mu g mL(-1) with hydrodynamic injectio
n, and from 0.08 to 0.16 mu g mL(-)1 with electrokinetic injection; injecti
on reproducibility (RSD %) was better with pressure injection (4.7-7.9 %) t
han with electrokinetic injection (8.6-20.7 %). The method was applied to t
he analysis of real and spiked samples, recoveries ranged from 6 % for PHIP
to 91 % for 4,8-DiMeIQx from various cooked foods.