Analysis of cannabinoids in fiber hemp plant varieties (Cannabis sativa L.) by high-performance liquid chromatography

An analytical procedure was developed for the detection of neutral and acid ic cannabinoids in herbal cannabis without the need of any preliminary deri vatization. The method was used to assay cannabinoid content of over one hu ndred fiber hemp samples grown in different Italian localities and harveste d at different maturation level degrees during the summer. No interferences were observed due to the vegetal matrix. The influence of genetic factors and environmental conditions on cannabinoi d content is discussed; the results may be of interest to enhance potential of fiber hemp in compliance with law enforcement purposes.