Preparation and characterization of p-tert-butyl-calix[6]arene-bonded silica gel stationary phase for high-performance liquid chromatography

For the first time calix[6]arene has been chemically combined with silica g el via a longer spacer to prepare calix[6]arene-bonded silica gel stationar y phase for high-performance liquid chromatography (HPLC). The separation o f positional isomers and polycyclic aromatic hydrocarbons on the calix[6] a rene-bonded phase was achieved with methanol-water as mobile phase. Some nu cleosides were also separated on the bonded phase. The reversed-phase chrom atographic performance of the bonded phase was studied. The results showed that the calix[6]arene-bonded phase was highly hydrophobic A possible separ ation mechanism has been proposed.