Jm. Lin et al., Enantiomeric resolution of dansyl amino acids by capillary electrochromatography based on molecular imprinting method, CHROMATOGR, 47(11-12), 1998, pp. 625-629
Recognition sites incorporating both pyridinyl and carboxy functionalities
prepared by molecular imprinting were used for the enantioseparation of dan
sylated amino acids by capillary electrochromatography (CEC). Simultaneous
non-covalent molecular imprinting of two chemically distinct functional mon
omers, vinylpyridine and methacrylic acid, was demonstrated in ethylene gly
col dimethacrylate-based copolymers. Polymers obtained were ground and siev
ed to particles < 10 mu m for filling into capillaries for CEC. Such imprin
ted polymers demonstrated improved recognition capabilities compared to pol
ymers incorporating only one of the functional monomers. Several aspects of
polymer preparation were investigated including the polymerization tempera
ture, effect of solvent, composition of polymer together with the running c
onditions of the CEC. Separation was highly-specific and depended on the pr
esence of both print molecule and functional monomers in the polymerization
mixture.