Av. Osadchuk et Kv. Svechnikov, The genetic control of the activities of microsomal steroidogenic enzymes in Leydig cells of inbred strains of mice, GENETIKA, 34(9), 1998, pp. 1277-1285
Pour activities of key microsomal steroidogenjc enzymes (3 beta-hyaroxyster
oid dehydrogenase-isomerase, microsomal cytochrome P450(17 beta), and 17-ke
tosteroid reductase) were compared in Leydig cells of six inbred strains of
mice: A/He, CBA/Lac, C57BL/6J, DD, YT, and PT. The activities of the enzym
es were found to vary considerably from one strain to another, depending on
the genotype, Analysis of testosterone biosynthesis revealed a decreased a
ctivity of 17-ketosteroid reductase as compared to the activities of other
microsomal steroidogenic enzymes, The metabolism of pregnenolone and proges
terone demonstrated features dependent on the genotype and caused by genoty
pic differences in the activities of microsomal steroidogenic enzymes. Thes
e activities demonstrated a correlative interstrain variation indicative of
their coordinated genetic control. The correlative variation may be relate
d to a major gene effect on the activities of microsomal steroidogenic enzy
mes.