Genetic control of molecular forms of alcohol dehydrogenase, glutamate dehydrogenase, isocitrate dehydrogenase, and NAD-dependent malate dehydrogenase in buckwheat Fagopyrum esculentum Moench

An electrophoretic analysis of isozymes of alcohol dehydrogenase (ADH), glu tamate dehydrogenase (GDH), isocitrate dehydrogenase (IDH), and NAD-depende nt malate dehydrogenase (NAD-MDH) was conducted, using a collection of buck wheat homostylous lines and cultivars-populations. The enzymes of different tissues-seeds, nondifferentiated seedlings, leaves, and generative organs were studied, A relatively high activity in the assayed enzymes was noted. A small variation in the enzymes' molecular forms was observed. All inbred lines of the collection were monomorphic for all enzymes studied. In three populations, Veselka, Viktoriya, and Zelenotsvetkovaya 90, slight polymorph ism for the isozyme patterns of ADH, GDH, and IDH was detected. The genetic control of ADH and IDH was studied. It was shown that ADH is controlled by one Adh locus with alleles Adh-F and Adh-S, while IDH is controlled by two loci: Idhl, with alleles Idhl-F and Idhl-S, and a monomorphic Idh2 locus. We failed to determine the genetic control of GDH, since plants carrying th e rare allele were less viable and were eliminated from the population befo re the formation of generative organs. NAD-MDH was controlled by three loci , all of which were monomorphic.