Distribution of T-bands on mitotic chromosomes of Bos taurus was studied. A
ssociation of T-bands with telomeres and enrichment of T-bands with genes,
with a known localization is described, After THA-banding on the chromosome
s of cattle, telomeric and pericentromeric regions of all autosomes showed
bright fluorescence. The exception was for chromosome 7, which did not have
telomeric T-bands, Interstitial T-bands were detected only on chromosomes
7, 16, and Y (7q13, 7q15, 7q22, 7q24, 16q21, and Yp12), A total proportion
of centromeric, telomeric, and interstitial T-bands was 11.19, 9.97, and 2.
02% of the length of the haploid chromosome set, respectively, By means of
fluorescent in situ hybridization (FISH), the presence of the telomeric rep
eat (TTAGGG)(n) was shown not only in telomeric regions of all autosomes, b
ut also in all pericentromeric regions. The obtained data are indicative of
the specificity of T-banding on the chromosomes of Bos taurus.