A species-specific monoclonal antibody to Cynodon dactylon

Background: It is usually difficult to differentiate between the pollens of different grass species on the basis of their appearance under a microscop e, as they often appear similar. Such distinctions are important, when inte rpreting the clinical relevance of pollens in air samples as individuals ca n differ in their allergic responses to different grass species. As this al lergenic distinction occurs at the level of presence and differences of epi topes on the allergens associated with different species, it could be antic ipated that species-specific monoclonal antibody could provide such distinc tions between pollens. Method: Monoclonal antibodies raised against Cynodon dactylon were screened and characterised in ELISA assays and blotting, usi ng a range of grass pollen,extracts, to identify clones which were species specific. Results: The most specific monoclonal raised to C. dactylon did n ot react at a level of greater than 1.2% to extracts of 10 other grass poll ens in a direct ELISA assay and showed no detectable cross-reactivity in a particle blotting assay. Conclusion: It has been possible to produce a mono clonal antibody that is functionally species specific to C. dactylon.