Ra. Norman et al., Absence of genetic variation in some obesity candidate genes (GLP1R, ASIP,MC4R MC5R) among Pima Indians, INT J OBES, 23(2), 1999, pp. 163-165
OBJECTIVE: To examine the obesity candidate genes glucagon-like-peptide rec
eptor (GLP1R), agouti signaling protein (ASlP) and the melanocortin recepto
rs 4 and 5 (MC4R and MC5R) for DNA polymorphisms in their coding regions.
SUBJECTS: Unrelated, non-diabetic Pima Indians (8 to 12 from each extreme o
f body fat).
MEASUREMENTS: DNA sequencing within the coding regions of each gene. RESULT
: Only one variant was detected, a silent substitution in exon 6 of GLP1R.
CONCLUSION: The exclusion of any common amino-acid polymorphisms (allele fr
equency greater than or equal to 0.20). implies that structural variants of
these genes do not contribute to variation in the high level of obesity ob
served among the Pima Indians.