PRELIMINARY-X-RAY DIFFRACTION STUDIES ON ASPARAGINYL-TRANSFER-RNA SYNTHETASE FROM THERMUS-THERMOPHILUS

Authors
BERTHETCOLOMINAS C SEIGNOVERT L CUSACK S LEBERMAN R
Citation
C. Berthetcolominas et al., PRELIMINARY-X-RAY DIFFRACTION STUDIES ON ASPARAGINYL-TRANSFER-RNA SYNTHETASE FROM THERMUS-THERMOPHILUS, Acta crystallographica. Section D, Biological crystallography, 53, 1997, pp. 195-196
Citations number
13
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biology
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
53
Year of publication
1997
Part
2
Pages
195 - 196
Database
ISI
SICI code
0907-4449(1997)53:<195:PDSOAS>2.0.ZU;2-L
Abstract
The recombinant asparginyl-tRNA synthetase from the thermophilic bacte rium Thermus thermophilus expressed in Escherichia coli has been cryst allized from PEG 6000 solutions. Depending on the PEG concentrations t he crystals were in either tetragonal or hexagonal space groups. Altho ugh generally smaller, the latter (space group P6(4)22) diffracted bet ter, to a resolution of 2.8 Angstrom. Using the coordinates of the yea st aspartyl-tRNA synthetase structure molecular replacement methods we re applied to both tetragonal and hexagonal crystals; a solution was f ound which gave excellent crystal packing in both space groups.