J. Vincentelli et al., Evaluation of the polyethylene glycol-KF-water system in the context of purifying PEG-protein adducts, INT J PHARM, 176(2), 1999, pp. 241-249
Covalent binding of PEG to proteins leads to conjugates widely investigated
in several biotechnological processes. Their use as pharmaceuticals requir
es both careful purification and proper characterization. In this context,
this paper examines the potentialities offered by hydrophobic interaction c
hromatography and compares aqueous potassium fluoride and ammonium sulfate
as the eluents. Relative contribution of the various forces which dictate t
he chromatographic behaviour of PEG-protein adducts on Fractogel TSK-Butyl
650 is discussed. (C) 1999 Elsevier Science B.V. All rights reserved.