CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF THE FIMC-FIMH CHAPERONE-ADHESIN COMPLEX FROM ESCHERICHIA-COLI

Authors
KNIGHT S MULVEY M PINKNER J
Citation
S. Knight et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF THE FIMC-FIMH CHAPERONE-ADHESIN COMPLEX FROM ESCHERICHIA-COLI, Acta crystallographica. Section D, Biological crystallography, 53, 1997, pp. 207-210
Citations number
20
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biology
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
53
Year of publication
1997
Part
2
Pages
207 - 210
Database
ISI
SICI code
0907-4449(1997)53:<207:CAPDSO>2.0.ZU;2-N
Abstract
A complex of the periplasmic chaperone FimC and the mannose-binding ad hesin FimH from the Escherichia coli type 1 pilus system has been crys tallized from ammonium sulfate solution using the hanging-drop vapour- diffusion method. The crystals diffract to a minimum Bragg spacing of 2.7 Angstrom and belong to the space group P4(1)2(1)2 or P4(3)2(1)2 wi th cell dimensions a = b = 97.7, c = 215.9 Angstrom at room temperatur e. Data to 3.0 Angstrom have been collected from a single crystal froz en to T = 100K.