U. Behrendt et al., A taxonomic study of bacteria isolated from grasses: a proposed new species Pseudomonas graminis sp. nov., INT J SY B, 49, 1999, pp. 297-308
The taxonomic position of a yellow-pigmented group of bacteria, isolated fr
om the phyllosphere of grasses was investigated. Results obtained from rest
riction analysis of amplified 16S rDNA with seven endonucleases (Cfol, HaeI
II, AluI, HinfI, MspI, Sau3A and ScrFI) showed identical restriction patter
ns for each enzyme of all isolates studied, which suggests that all strains
belong to the same species. The grass isolates displayed the characteristi
cs of the genus Pseudomonas. They were Gram-negative, aerobic and rod-shape
d with polar flagella. Isolates were catalase-positive and oxidase-negative
, and unable to oxidize or ferment glucose with the production of acid. The
isolates did not reduce nitrate to nitrite but were able to utilize a wide
range of compounds individually as a sole carbon source, with preference b
eing given to the utilization of monosaccharides. The disaccharides tested
were not utilized as substrates. The DNA base compositions of the tested st
rains ranged from 60 to 61 mol% G+C. The major isoprenoid quinone of each w
as ubiquinone Q-9 and hydroxy fatty acids were represented by 3-hydroxydode
canoic acid and 2-hydroxydodecanoic acid. Comparison of 16S rDNA sequences
showed that the bacteria were members of the genus Pseudomonas, with simila
rity values between 91.5 and 97.7 %. DNA-DNA hybridization studies with clo
sely related neighbours revealed a low level of homology (< 27 %), indicati
ng that the isolates represent an individual species. On the basis of pheno
typic and phylogenetic analyses a new species, Pseudomonas graminis sp. nov
. (type strain DSM 11363(T)), is proposed.