Six 100 ppm fumonisin B-1 (FB1) solutions were prepared by dissolving pure
standard in six different solvents containing SO2. Two of the solvents cont
ained 0.2 or 0.4% SO2 in distilled water. The other four solvents were obta
ined by steeping corn kernels at 60 degrees C in a 0.2% SO2 aqueous solutio
n for 6, 12, 24, or 48 h. After the addition of FB1, all solutions were mai
ntained at 60 degrees C for 7 days. Fumonisin B-1 content in each solution
was determined in triplicate by HPLC. Steeping corn kernels in 0.2% solutio
n at 60 degrees C for 6 h seems to be the most effective treatment to decre
ase the amount of FB1.