Carbon paste biosensor based on crude soybean seed hull extracts for phenol detection

In this study, a novel biosensor based on enzyme extracts from soybean seed hulls has been prepared, which demonstrated promising results in the detec tion of hydrogen peroxide and phenol. The biosensor preparation is straight forward and inexpensive, and the response time is 50 s. The optimum conditi ons of pH and temperature are a pH of 7.4 and a temperature of 20 degrees C , Contrary to expectations, the biosensor showed narrow pH and temperature optimums. The effects of enzyme loading and type of mediator were also inve stigated. The biosensor showed a linear response up to 500 mu M phenol.