Merrifield resin and 1,6-hexanediol diacrylate-crosslinked polystyrene resin for solid-phase peptide synthesis: A comparative study

Three short peptides were synthesized on Merrifield resin (PS-DVB) and on p olystyrene crosslinked with 1,6-hexanediol diacrylate resin (PS-HDODA) to c ompare their efficiencies in solid-phase peptide synthesis (SPPS). A 2% cro sslinked polymeric system having almost equal capacity was used in both cas es. The peptides were synthesized using standard solid-phase methodology. I n the case of the PS-HDODA resin, all the couplings were completed by the f irst coupling and the peptides were obtained in > 90% yield and > 95% purit y. But in the case of the PS-DVB resin, most of the attachments require two to three couplings and the peptides were obtained in about a 65% yield. (C ) 1999 John Wiley & Sons, Inc.