The main purpose of this laboratory study was to determine the tooth whiten
ing effect of a baking soda dentifrice containing stabilized 1% hydrogen pe
roxide Following repeated applications to intrinsically stained teeth. Addi
tional objectives were to investigate (1) the influence of tooth surface cu
rvature and moisture on color measurements, (2) preliminary treatment param
eters using 1% hydrogen peroxide solution, and (3) the relationship between
Vita(R) shade guide standards and instrumental color readings. Human tooth
specimens with intrinsic stain were specially prepared and measured for co
lor (L*a*b*) by means of diffuse reflectance spectrophotometry. Specimens w
ere measured under wet and dry conditions with natural and flattened enamel
surfaces. A preliminary test was conducted with 1% H2O2 solution to establ
ish tooth whitening versus treatment time. Then, new tooth specimens were t
reated repeatedly with slurries of silica, baking soda, or baking soda/pero
xide dentifrices at 30-minute intervals, and color measurements were taken
under both wet and dry conditions after 4, 8, 16 and 24 hours. L*a*b* color
measurements of the Vita(R) shade guide teeth were also made in order to r
elate laboratory color changes to clinical effects. The parameter testing r
esults showed that flattening the enamel surface had no effect on tooth col
or, but the teeth rapidly became lighter and less yellow upon air-drying. T
he 1% H2O2 solution caused both a significant decrease in yellow (b*) and a
n increase in lightness (L*). Since the reduction for b* occurred more rapi
dly and to a greater extent than the L* factor, the b* component may be the
more important indicator of tooth whitening by bleaching. Compared to the
silica and baking soda dentifrices, the baking soda/peroxide dentifrice sig
nificantly decreased the yellow color (b*) of the teeth after 8 or more hou
rs of topical treatment. The instrumental color readings of the Vita Shade
guide teeth showed that the color differences between the standards were no
t linear or consistent, and their accuracy for assessing clinical changes i
n tooth whiteness is questionable.