Test characteristics of acridine orange, gram, and May-Grunwald-Giemsa stains for enumeration of intracellular organisms in bronchoalveolar lavage fluid
E. De Brauwer et al., Test characteristics of acridine orange, gram, and May-Grunwald-Giemsa stains for enumeration of intracellular organisms in bronchoalveolar lavage fluid, J CLIN MICR, 37(2), 1999, pp. 427-429
For enumeration of intracellular organisms (ICO) in bronchoalveolar lavage
fluid samples, the May-Grunwald-Giemsa (MGG) stain displayed higher interob
server agreement than the acridine orange and Gram stains. The MGG stain of
fered a reliable enumeration of ICO when 200 cells were counted by one obse
rver.