PCR-enzyme-linked immunosorbent assay for detection and identification of Campylobacter species: Application to isolates and stool samples

Authors
Metherell, LA Logan, JMJ Stanley, J
Citation
La. Metherell et al., PCR-enzyme-linked immunosorbent assay for detection and identification of Campylobacter species: Application to isolates and stool samples, J CLIN MICR, 37(2), 1999, pp. 433-435
Citations number
8
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
37
Issue
2
Year of publication
1999
Pages
433 - 435
Database
ISI
SICI code
0095-1137(199902)37:2<433:PIAFDA>2.0.ZU;2-#
Abstract
We report a PCR-enzyme-linked immunosorbent assay which identifies Campylob acter species by capture hybridization of a single-stranded 16S rRnA gene a mplicon with species-specific probes in a microtiter plate format. Specific ities were confirmed for both reference and field strains, but the type str ain of Campylobacter coli was atypical. The assay was rapid, informative, a nd usable with stool-extracted DNA.