Gram type-specific broad-range PCR amplification for rapid detection of 62pathogenic bacteria

Authors
Klausegger, A Hell, M Berger, A Zinober, K Baier, S Jones, N Sperl, W Kofler, B
Citation
A. Klausegger et al., Gram type-specific broad-range PCR amplification for rapid detection of 62pathogenic bacteria, J CLIN MICR, 37(2), 1999, pp. 464-466
Citations number
8
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
37
Issue
2
Year of publication
1999
Pages
464 - 466
Database
ISI
SICI code
0095-1137(199902)37:2<464:GTBPAF>2.0.ZU;2-X
Abstract
Broad-range PCR has proven to be useful for the detection of bacteria. A se t of broad-range PCR primers directed against conserved regions in the 16S rRNA gene was designed to specifically amplify either grampositive or gram- negative bacteria. The gram type-specific broad-range PCR correctly classif ied all 62 pathogenic species tested.