Quantitation of human immunodeficiency virus type 2 DNA in peripheral blood mononuclear cells by using a quantitative-competitive PCR assay

Authors
Gomes, P Taveira, NC Pereira, JM Antunes, F Ferreira, MOS Lourenco, MH
Citation
P. Gomes et al., Quantitation of human immunodeficiency virus type 2 DNA in peripheral blood mononuclear cells by using a quantitative-competitive PCR assay, J CLIN MICR, 37(2), 1999, pp. 453-456
Citations number
24
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
37
Issue
2
Year of publication
1999
Pages
453 - 456
Database
ISI
SICI code
0095-1137(199902)37:2<453:QOHIVT>2.0.ZU;2-V
Abstract
A new quantitative-competitive PCR-based human immunodeficiency virus type 2 (HIV-2) proviral DNA assay (QC-PCR) was developed and used to determine t he proviral load in HIV-2-infected individuals. Proviral load varied consid erably, with means of 1,831 copies per 10(6) peripheral blood mononuclear c ells for asymptomatic subjects (n = 19) and 2,587 for AIDS patients (n = 2) . HIV-2 viral and proviral loads also varied significantly over time in asy mptomatic patients. These data suggest that a high level of virus replicati on occurs throughout the asymptomatic phase of HIV-2 infection.