Detection of Cryptosporidium parvum in horses: Thresholds of acid-fast stain, immunofluorescence assay, and flow cytometry

Authors
Cole, DJ Snowden, K Cohen, ND Smith, R
Citation
Dj. Cole et al., Detection of Cryptosporidium parvum in horses: Thresholds of acid-fast stain, immunofluorescence assay, and flow cytometry, J CLIN MICR, 37(2), 1999, pp. 457-460
Citations number
10
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
37
Issue
2
Year of publication
1999
Pages
457 - 460
Database
ISI
SICI code
0095-1137(199902)37:2<457:DOCPIH>2.0.ZU;2-#
Abstract
Feces collected from three asymptomatic horses and seeded with Cryptosporid ium parvum oocysts (10(1) to 10(6)/g of feces) were evaluated by acid-fast staining (AF), an immunofluorescent antibody (IFA) technique, and flow cyto metry. The thresholds of detection were 5 x 10(5) oocysts/g of feces for th e IFA and AF techniques and 5 x 10(4) oocysts/g for flow cytometry.