Characterization of two interspecific hybrids of Piper

Two interspecific hybrids of Piper, P nigrum x P. attenuatum and P. nigrum x P. barberi, produced for the first time, were characterized by morphology , anatomy, isozymes, cytology and function (reaction to pollu beetle). The hybrids exhibit distinct morphological and anatomical features. Hybrid-spec ific bands as well as male-specific bands were observed in the zymograms of the isoforms of three of the four isozymes, peroxidase, esterase and polyp henol oxidase. Paired affinity index of the four enzymes revealed more simi larity between the female parents and hybrids than between the male parents and hybrids. The hybrids had the same chromosome number (2n = 52) as their parents. The leaves of the hybrids were less preferred for feeding by poll u beetles when compared with their female parents. Successful hybridization among the three species belonging to the same subgenus Maricha confirms th eir phylogenic relationship.