M. Wu et al., SOLUTION STRUCTURE OF (RGGC(AG)UNDER-BAR-GCC)(2) BY 2-DIMENSIONAL NMRAND THE ITERATIVE RELAXATION MATRIX APPROACH, Biochemistry, 36(15), 1997, pp. 4449-4460
The three-dimensional solution structure of the RNA self-complementary
duplex [GRAPHICS] was derived from two-dimensional NMR and the iterat
ive relaxation matrix approach. Each GA mismatch forms two hydrogen bo
nds: A-NH6 to G-O6 and A-N1 to G-NH1 (imino). This GA structure differ
s from the sheared tandem GA structure in [GRAPHICS] which also has tw
o hydrogen bonds: A-N7 to G-NH2 and A-NH6 to G-N3 [SantaLucia, J., Jr.
, & Turner, D. H. (1993) Biochemistry 32, 12612-12623], although the o
nly difference between the two sequences is the order of the two GA mi
smatches. Inspection of three-dimensional structures indicates that su
bstituting [GRAPHICS] for [GRAPHICS] makes GA mismatches unable to hav
e stable sheared conformations. This may explain why the [GRAPHICS] mo
tif is rarely observed in nature, whereas [GRAPHICS] is common.