STRUCTURE OF THE CAPSULAR POLYSACCHARIDE FROM ALTEROMONAS-NIGRIFACIENS IAM 13010T CONTAINING 2-ACETAMIDO-2,6-DIDEOXY-L-TALOSE AND 3-DEOXY-D-MANNO-OCTULOSONIC ACID

A capsular polysaccharide was obtained from Alteromonas nigrifaciens I AM 13010T by saline extraction. On the basis of H-1 and C-13 NMR spect roscopy, including one-dimensional (1D) NOE spectroscopy, 2D rotating- frame NOE spectroscopy (ROESY), and H-1-detected heteronuclear H-1, C- 13 multiple-quantum coherence (HMQC), it was concluded that the polysa ccharide contained inter alia an acidic sugar, 3-deoxy-D-manno-octulos onic acid (Kdo), and a rare amino sugar, 2-acetamido-2,6-dideoxy-L-tal ose (L-6dTalNAc, N-acetylpneumosamine), and has a pentasaccharide repe ating unit of the following structure: [GRAPHICS] (C) 1997 Elsevier Sc ience Ltd.