Evaluation of 8-oxodeoxyguanosine, typical oxidative DNA damage, in lymphocytes of ozone-treated arteriosclerotic patients

In the present study we measured the amount of 8-oxo-2'-deoxyguanosine (8-o xo-dG) in DNA isolated from lymphocytes of arteriosclerotic patients underg oing ozonetherapy. Treatment of the patients with therapeutic concentration of ozone caused a significant increase over the control value in the amoun t of 8-oxo-dG of DNA isolated from their lymphocytes. However, only three o ut of six patients examined responded positively to the treatment in terms of the base damage. The increases varied among patients, and were in the ra nge of 100-450%. This interindividual difference may at least be partly exp lained by recently demonstrated heritable susceptibility to ozone. (C) 1999 Elsevier Science B.V. All rights reserved.