EFFICIENCY OF CHEMICAL OLIGONUCLEOTIDE SYNTHESIS EVALUATED BY ION-EXCHANGE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Ion-exchange high-performance liquid chromatography (HPIEC) is a metho d of choice for the separation of crude solid-phase synthesized oligon ucleotides. It shows peaks for target and error sequences and it is us ed for quantification of oligonucleotide synthesis. This paper present s a homeodynamic model of polymer-supported oligonucleotide synthesis and a numerical solution, which accounts for elution profiles obtained by HPIEC. The elution profiles are consistent with the model. The cha in-length distributions for 30mer syntheses on different support mater ials were also studied and the probability moment analysis was applied as a quantitative measure of the differential relationship of fractal dimension. The support systems for chemical synthesis of short oligon ucleotides are classified according to this relationship.