A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells

Authors
Piechaczek, C Fetzer, C Baiker, A Bode, J Lipps, HJ
Citation
C. Piechaczek et al., A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells, NUCL ACID R, 27(2), 1999, pp. 426-428
Citations number
18
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEIC ACIDS RESEARCH
ISSN journal
03051048 → ACNP
Volume
27
Issue
2
Year of publication
1999
Pages
426 - 428
Database
ISI
SICI code
0305-1048(19990115)27:2<426:AVBOTS>2.0.ZU;2-C
Abstract
We have developed an episomal replicating expression vector in which the SV 40 gene coding for the large T-antigen was replaced by chromosomal scaffold /matrix attached regions. Southern analysis as well as vector rescue experi ments in CHO cells and in Escherichia coli demonstrate that the vector repl icates episomally in CHO cells. it occurs in a very low copy number in the cells and is stably maintained over more than 100 generations without selec tion pressure.