Different polypeptide profiles from tomato roots following interactions with arbuscular mycorrhizal (Glomus mosseae) or pathogenic (Phytophthora parasitica) fungi

In order to investigate the synthesis of arbuscular mycorrhiza-related poly peptides, protein extracts from tomato roots colonized with the arbuscular mycorrhizal fungus Glomus mosseae were analysed by two-dimensional gel elec trophoresis. Polypeptide patterns of mycorrhizal tomato roots were compared not only to those of non-infected ones, but also to those of tomato roots infected with the pathogenic fungus Phytophthora parasitica. Comparisons of the various polypeptide profiles showed that additional polypeptides were induced specifically in response to arbuscular mycorrhizal symbiosis versus pathogenic interaction. Moreover, comparison of polypeptide: patterns of G . mosseae-colonized and P. parasitica-infected tomato roots with those of e ither germinated spores of the arbuscular mycorrhizal fungus or of axenical ly grown pathogenic fungus, suggested that some of the additional polypepti des were of plant origin.