Molecular cloning and nucleotide sequence of 3 '-terminal region of classical swine fever virus LPC vaccine strain

A cDNA of the 3'-terminus of classical swine fever virus (LPC vaccine strai n) was cloned and sequenced. The 3431 nucleotides and deduced amino acid se quences were compared with those of other pestiviruses, and the similarity of nucleotide sequences and deduced amino acid sequences were found to be 8 4-95% and 95-98%, respectively. Similar to other isolates of classical swin e fever virus, the sequenced region included the non-structural gene p58 (N S5A) and part of p76 (NS5B) gene. The p76 gene of LPC vaccine strain also c ontained a highly conserved motif G-D-D (Gly-Asp-Asp) that is present in th e RNA replicase of positive-stranded RNA viruses. With the sequence data cu rrently available, we carried out a phylogenetic analysis and obtained a ge nealogical relationship among members of the classical swine fever virus. T he sequence has been submitted to GeneBank with an accession number AF00198 6.