We investigated the influence of flood-induced channel changes on the hypor
heic zone of 4th- and 5th-order reaches of a mountain stream network. Prefl
ood versus postflood comparisons were made in three study reaches from well
networks established before and reestablished after a major flood. Flood e
ffects were scale dependent and varied with channel constraint and the domi
nant channel forming process. Large changes were observed in unconstrained
stream reaches where channel incision drove large changes in subsurface flo
w paths and the extent of the hyporheic zone. However, subreach scale diffe
rences were apparent. In the lower portion of the studied reach, channel in
cision lowered the water table, leading to abandonment of secondary channel
s, and decreased the extent of the hyporheic zone that previously extended
more than 30 m into the floodplain. In contrast, the extent of the hyporhei
c zone increased at the head of the studied reach where channel incision st
eepened head gradients through a meander bend. In another unconstrained rea
ch, lateral channel jumps dramatically altered exchange flow paths. However
, the extensive hyporheic zone was maintained throughout the reach. Less ch
ange was observed in the constrained stream reach where both the depth and
area of sediment available to be reworked by the flood were limited by bedr
ock constraining the width of the valley floor. This flood dramatically cha
nged the hyporheic zone at the three study sites and these physical changes
are expected to be biologically important, considering the role of the hyp
orheic zone in stream ecosystem processes.