Murine class I major histocompatibility complex H-2D(d): expression, refolding and crystallization

Authors
Achour, A Harris, RA Persson, K Sundback, J Sentman, CL Schneider, G Lindqvist, Y Karre, K
Citation
A. Achour et al., Murine class I major histocompatibility complex H-2D(d): expression, refolding and crystallization, ACT CRYST D, 55, 1999, pp. 260-262
Citations number
31
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
55
Year of publication
1999
Part
1
Pages
260 - 262
Database
ISI
SICI code
0907-4449(199901)55:<260:MCIMHC>2.0.ZU;2-E
Abstract
A truncated soluble form of the murine class I major histocompatibility ant igen complex H-2D(d) was cloned using an Escherichia coli based system. It was expressed, refolded in vitro and crystallized in a complex with murine beta(2) microglobulin and the peptide RGPGRAFVTI from the V3-loop of the gp 160 HIV-1 protein. Crystals belonging to the space group P2(1)2(1)2(1) with cell dimensions a = 51.3, b = 92.5, c = 108.8 Angstrom were obtained using two different crystallization conditions. The crystals contain one complex per asymmetric unit and diffract to at least 2.4 Angstrom resolution.