Crystallization and preliminary X-ray data for the human transthyretin-retinol-binding protein (RBP) complex bound to an anti-REP Fab

A macromolecular complex of human transthyretin, human retinol-binding prot ein and an anti-retinol-binding-protein Fab was crystallized by vapour diff usion in sitting drops. Diffraction from these crystals at cryogenic temper atures was consistent with the space group C222, with cell parameters a = 1 59.34, b = 222.40 and c = 121.27 Angstrom. Crystals diffracted to a resolut ion limit of 3.36 Angstrom using synchrotron radiation. Based on a 2:2:1 st oichiometry for the Fab-retinol-binding-protein-transthyretin complex and t he presence of one such complex per asymmetric unit, a reasonable V-m coeff icient of 2.74 Angstrom(3) Da(-1) could be estimated.