Expression, purification, crystallization and preliminary X-ray diffraction results from Campylobacter jejuni ferritin

Authors
Clerte, S Dautant, A d'Estaintot, BL Gallois, B Mizunoe, Y Wai, SN Precigoux, G
Citation
S. Clerte et al., Expression, purification, crystallization and preliminary X-ray diffraction results from Campylobacter jejuni ferritin, ACT CRYST D, 55, 1999, pp. 299-301
Citations number
20
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
55
Year of publication
1999
Part
1
Pages
299 - 301
Database
ISI
SICI code
0907-4449(199901)55:<299:EPCAPX>2.0.ZU;2-A
Abstract
The prokaryotic ferritin gene of Campylobacter jejuni was overexpressed in Escherichia coli under control of the bacteriophage T7 promoter and the pro tein (Cj-FTN) purified. Preliminary crystallization experiments have been p erformed using the hanging-drop vapour-diffusion method with ammonium sulfa te as the precipitant. Diffraction studies show the crystals belong to the I432 space group (a = 151.52 Angstrom). Structure solution by molecular rep lacement is in progress while crystal quality improvement is carried out.