Crystallization and preliminary X-ray diffraction studies of phospholipaseD from Streptomyces antibioticus

Phospholipase D (E.C. 3.1.4.4) from Streptomyces antibioticus has been crys tallized in six crystal forms using the hanging-drop vapour-diffusion metho d. The type III and V crystals belong to monoclinic and hexagonal systems, respectively All of the other crystal forms, types I, II, IV and VI, belong to orthorhombic space group P2(1)2(1)2(1) Of these four types, the type VI crystals are suitable for X-ray structure determination. Crystal data for type VI crystals are: a = 50.1, b = 98.7, c = 107.6 Angstrom, V = 532100 An gstrom(3), Z = 4 and V-m = 2.47 Angstrom(3) Da(-1) Type VI crystals diffrac t to at least 2.3 Angstrom resolution. A total of 11295 independent reflect ions to 3 Angstrom resolution have been collected from a type VI crystal us ing a conventional X-ray source, and its structural analysis is currently b eing conducted using isomorphous replacement methods.