M. Knezevic et al., Light microscopic and immunohistochemical investigation of Peyers patches in calves experimentally infected with rotavirus, ACT VET BEO, 48(5-6), 1998, pp. 331-337
Pathomorphological and immunohistochemical examinations were carried out on
tissue sections of the jejunum and ileum from calves experimentally infect
ed with rota virus immediately after birth. Rota virus particles were prese
nt in the gut lumen in absorptive enterocytes next to, and in the direct vi
cinity of dome epithelial cells, but were never seen inside these cells. Th
us, dome epithelial cells did not reveal any participation in rota virus in
fection. In this work, we demonstrate that a human antiserum recognizing a
phylogenetically conserved part of the CD3 epsilon cytoplasmatic tail detec
ts T cells of calves in formalid-fixed paraffin-embedded tissue sections. T
his antiserum enables the morphological characterization of normal and path
ological lymphoid tissues and lymphoid infiltrations in experimental work a
nd in animal disease.