Light microscopic and immunohistochemical investigation of Peyers patches in calves experimentally infected with rotavirus

Pathomorphological and immunohistochemical examinations were carried out on tissue sections of the jejunum and ileum from calves experimentally infect ed with rota virus immediately after birth. Rota virus particles were prese nt in the gut lumen in absorptive enterocytes next to, and in the direct vi cinity of dome epithelial cells, but were never seen inside these cells. Th us, dome epithelial cells did not reveal any participation in rota virus in fection. In this work, we demonstrate that a human antiserum recognizing a phylogenetically conserved part of the CD3 epsilon cytoplasmatic tail detec ts T cells of calves in formalid-fixed paraffin-embedded tissue sections. T his antiserum enables the morphological characterization of normal and path ological lymphoid tissues and lymphoid infiltrations in experimental work a nd in animal disease.