Cell ashing for trace element analysis: A new approach based on ultraviolet/ozone

Authors
De Stasio, G Gilbert, B Perfetti, L Hansen, R Mercanti, D Ciotti, MT Andres, R White, VE Perfetti, P Margaritondo, G
Citation
G. De Stasio et al., Cell ashing for trace element analysis: A new approach based on ultraviolet/ozone, ANALYT BIOC, 266(2), 1999, pp. 174-180
Citations number
17
Categorie Soggetti
Biochemistry & Biophysics
Journal title
ANALYTICAL BIOCHEMISTRY
ISSN journal
00032697 → ACNP
Volume
266
Issue
2
Year of publication
1999
Pages
174 - 180
Database
ISI
SICI code
0003-2697(19990115)266:2<174:CAFTEA>2.0.ZU;2-B
Abstract
We studied a new approach to cell ashing based on illuminating the specimen s with a low-pressure mercury discharge lamp. We analyzed with synchrotron spectromicroscopy its effects on different physiological elements in neurob iological specimens. Our results demonstrate that carbon is removed, wherea s phosphorus, calcium, potassium, and sulfur are retained and their relativ e concentrations are enhanced. Applied to trace elements, this technique wi ll enhance their practical detectability. (C) 1999 Academic Press.