Influence of various nucleotides on the in situ crystallization of Ca2+-ATPase

Authors
Hiroaki, Y Mitsuoka, K Cheng, YF Hiroaki, H Fujiyoshi, Y
Citation
Y. Hiroaki et al., Influence of various nucleotides on the in situ crystallization of Ca2+-ATPase, BBA-BIOMEMB, 1415(2), 1999, pp. 361-368
Citations number
21
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
ISSN journal
00052736 → ACNP
Volume
1415
Issue
2
Year of publication
1999
Pages
361 - 368
Database
ISI
SICI code
0005-2736(19990108)1415:2<361:IOVNOT>2.0.ZU;2-Z
Abstract
A reproducible in situ crystallization of the Ca2+-ATPase in isolated sarco plasmic reticulum (SR) membranes was studied. The addition of various nucle otides to the washing buffer allowed the formation of tubular crystals, whi ch is induced by vanadate. SR membranes washed with nucleotide-free buffer could not form tubular crystals upon subsequent incubation with vanadate. ( C) 1999 Elsevier Science B.V. All rights reserved.