Studies of chromophore retinal structure in bacteriorhodopsin by double-beam Fourier transform spectroscopy

Authors
Terpugov, EL Viskovatykh, AV Degtyareva, OV Fesenko, EE
Citation
El. Terpugov et al., Studies of chromophore retinal structure in bacteriorhodopsin by double-beam Fourier transform spectroscopy, BIOFIZIKA, 43(6), 1998, pp. 1002-1011
Citations number
39
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOFIZIKA
ISSN journal
00063029 → ACNP
Volume
43
Issue
6
Year of publication
1998
Pages
1002 - 1011
Database
ISI
SICI code
0006-3029(199811/12)43:6<1002:SOCRSI>2.0.ZU;2-B
Abstract
A new approach to studying chromophore-containing proteins is described, wh ich is based on double-beam Fourier transform spectroscopy. It is shown tha t dehydration changes the structure of the retinal of light-adapted purple membranes. Absorption spectra in infrared for the retinal of native bacteri orhodopsin (BR568), the first intermediate of the photocycle (K-610), and t he form BR506 in dry films were obtained. The spectra shown many new featur es compared with resonance Raman and FT-IR difference spectra.