Specificity of aspartic protease from fungus Trichoderma viride in the hydrolysis of oligopeptides

Authors
Simankova, AN Mirgorodskaya, OA Savel'eva, NV Savel'ev, AN Kerner, R Rijpstorff, P Alexandrov, SL
Citation
An. Simankova et al., Specificity of aspartic protease from fungus Trichoderma viride in the hydrolysis of oligopeptides, BIOORG KHIM, 24(11), 1998, pp. 822-830
Citations number
15
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANICHESKAYA KHIMIYA
ISSN journal
01323423 → ACNP
Volume
24
Issue
11
Year of publication
1998
Pages
822 - 830
Database
ISI
SICI code
0132-3423(199811)24:11<822:SOAPFF>2.0.ZU;2-U
Abstract
We isolated, purified, and characterized an aspartic protease from fungus T richoderma viride. The pH-dependence of the enzyme functioning was determin ed, and its specificity in the limited proteolysis of insulin and melittin was compared to the specificities of pepsin A and gastricsin. The kinetics of melittin hydrolysis by these enzymes was studied by mass spectrometry.