FACTORS CONTRIBUTING TO IMAGING OF XENOGRAFTS USING ANTI-PLACENTAL ALKALINE-PHOSPHATASE MONOCLONAL-ANTIBODY

Citation
K. Koshida et al., FACTORS CONTRIBUTING TO IMAGING OF XENOGRAFTS USING ANTI-PLACENTAL ALKALINE-PHOSPHATASE MONOCLONAL-ANTIBODY, The Journal of urology, 157(5), 1997, pp. 1941-1945
Citations number
19
Categorie Soggetti
Urology & Nephrology
Journal title
ISSN journal
00225347
Volume
157
Issue
5
Year of publication
1997
Pages
1941 - 1945
Database
ISI
SICI code
0022-5347(1997)157:5<1941:FCTIOX>2.0.ZU;2-2
Abstract
Purpose: To investigate factors that influence the imaging of placenta l alkaline phosphatase (FLAP) producing xenografts using an anti-FLAP monoclonal antibody (MAb). Materials and Methods: Three xenografts (hu man seminoma, HeLa Hep 2 cells, and KK-47 bladder cancer cells) each e xpressing FLAP to a different degree were used to immunolocalize an an ti-FLAP MAb, HPMS-1. Results: Although the highest FLAP level was foun d in seminoma xenografts, the MAb was not useful for the imaging of se minoma xenografts because of poor accumulation. Fragmentation of the M Ab, such as F(ab')2, however, was shown to be efficient for imaging se minoma xenografts. A distribution study with Tl-201 revealed the highe st blood flow in HeLa cells and the lowest in seminoma. A difference i n blood flow may partially explain the disparity between the amount of MAb accumulation and the level of antigen expression in these three x enografts. Conclusions: Blood flow in targeted tumors was shown to be more critical than their level of antigen expression for the imaging o f xenografts with anti-tumor antibody. In addition, fragmentation of t he MAb enabled tumor imaging because of a rapid clearance of the fragm ent from the circulation.