Determination of genotypic classes for trypsin inhibitor in soybean seeds

Authors
de Carvalho, WL Oliveira, MGD de Barros, EG Moreira, MA
Citation
Wl. De Carvalho et al., Determination of genotypic classes for trypsin inhibitor in soybean seeds, BIOTECH TEC, 12(12), 1998, pp. 859-863
Citations number
14
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOTECHNOLOGY TECHNIQUES
ISSN journal
0951208X → ACNP
Volume
12
Issue
12
Year of publication
1998
Pages
859 - 863
Database
ISI
SICI code
0951-208X(199812)12:12<859:DOGCFT>2.0.ZU;2-3
Abstract
An enzymatic non-destructive method was developed to determine the levels o f Kunitz Trypsin Inhibitor (KTI) in soybean seeds. The procedure was tested in F-2 seeds from crosses between a line lacking KTI (BRM) and six commerc ial varieties. The method allows identification of all three genotypic clas ses for KTI and will be extremely useful in breeding programs.