A competitive enzyme linked immunosorbent assay for recombinant Bm86 produced in Pichia pastoris

Authors
Reytor, E Hernandez, L Santana, H Blanco, R Barcelo, MT Rodriguez, I Boue, O
Citation
E. Reytor et al., A competitive enzyme linked immunosorbent assay for recombinant Bm86 produced in Pichia pastoris, BIOTECH TEC, 12(12), 1998, pp. 919-923
Citations number
8
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOTECHNOLOGY TECHNIQUES
ISSN journal
0951208X → ACNP
Volume
12
Issue
12
Year of publication
1998
Pages
919 - 923
Database
ISI
SICI code
0951-208X(199812)12:12<919:ACELIA>2.0.ZU;2-8
Abstract
A competitive enzyme linked immonosorbent assay (ELISA) was developed for q uantification of recombinant Bm86 protein (rBm86), antigen of the vaccine G avac(TM) against the cattle tick Boophilus microplus. Monoclonal antibody ( Mab) SSBm1 showed identical recognition to folded and denatured antigen and was used in the competition assay. This ELISA was sensitive enough to dete ct 60 ng/ml of rBm86. Within-assay coefficient of variation was 5.7 to 6.2 % and between-assay variation was 7.7 to 11.6 %. The level of expression of recombinant Bm86 in Pichia pastoris reached about 2.7 g per liter of cultu re after 80 hour of fermentation.