A reliable method for precise initial rate determination of enzyme activity under high hydrostatic pressure with simple apparatus - Application to Aspergillus niger fructosyl-transferase activity measurements

Citation
C. Vannieuwenburgh et al., A reliable method for precise initial rate determination of enzyme activity under high hydrostatic pressure with simple apparatus - Application to Aspergillus niger fructosyl-transferase activity measurements, BIOTECH TEC, 12(12), 1998, pp. 931-934
Citations number
12
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOTECHNOLOGY TECHNIQUES
ISSN journal
0951208X → ACNP
Volume
12
Issue
12
Year of publication
1998
Pages
931 - 934
Database
ISI
SICI code
0951-208X(199812)12:12<931:ARMFPI>2.0.ZU;2-I
Abstract
An original procedure for initial rate accurate determination of enzyme act ivity under high hydrostatic pressure is reported. This method, adapted to most kinds of enzyme systems, is based on the use of the linear property of product formation during the initial phase of a reaction and does not requ ire specific high pressure equipment. The reliability of the method was tes ted and illustrated with the study of Aspergillus niger fructosyl-transfera se activity as a function of substrate concentration above 300 MPa.