A reliable method for precise initial rate determination of enzyme activity under high hydrostatic pressure with simple apparatus - Application to Aspergillus niger fructosyl-transferase activity measurements

An original procedure for initial rate accurate determination of enzyme act ivity under high hydrostatic pressure is reported. This method, adapted to most kinds of enzyme systems, is based on the use of the linear property of product formation during the initial phase of a reaction and does not requ ire specific high pressure equipment. The reliability of the method was tes ted and illustrated with the study of Aspergillus niger fructosyl-transfera se activity as a function of substrate concentration above 300 MPa.