Antibodies with the cell-type specificity to the morula cells of the solitary ascidians styela rustica and boltenia echinata

Citation
Oi. Podgornaya et Tg. Shaposhnikova, Antibodies with the cell-type specificity to the morula cells of the solitary ascidians styela rustica and boltenia echinata, CELL STRUCT, 23(6), 1998, pp. 349-355
Citations number
35
Categorie Soggetti
Cell & Developmental Biology
Journal title
CELL STRUCTURE AND FUNCTION
ISSN journal
03867196 → ACNP
Volume
23
Issue
6
Year of publication
1998
Pages
349 - 355
Database
ISI
SICI code
0386-7196(199812)23:6<349:AWTCST>2.0.ZU;2-Q
Abstract
The separation of the blood cells of Styela rustica (Styelidae, Stolidobran chiae) in discontinuous Percoll gradient showed 4 fractions. The 4th and bo ttom most fraction contained 90-100% of morula cells. The protein compositi on of the morula cell fraction revealed on SDS-PAGE showed two major protei ns with m.w. 47 and 26 kDa. These proteins were heavily positively charged. Polyclonal antiserums against these proteins were raised. Each antiserum r eacted with both proteins only in morula cells on the blot after SDS-PAGE a nd stained the proper protein without crossreaction on the blot after AU-PA GE. The only type of cells stained with antibodies in circulating blood, in the tunic and on the tunic wound surface In paraffin sections of another s pecies Boltenia echinata (Pyuridae, Stolidobranchiae) were morula cells. Th e morula-type specific antibodies obtained recognized major positively char ged proteins which were apparently structural substrates for the phenoloxid ase tanning.