Studies on the resonant luminescence spectra of ethyl violet with deoxyribonucleic acid and its analytical application

The binding reaction and resonant luminescence spectra of ethyl violet with ctDNA have been studied, In the pH range of 5.0-10.0 ethyl violet exhibite d a maximum of absorption at 595 nm, A new absorption peak at 507 nm appear ed when ctDNA was added. It showed that a binding product of ethyl violet w ith DNA formed, The product emitted a sharp resonant luminescence peak at 5 10 nm, The intensity of resonant luminescence was found to Le dependent on the concentration of DNA, The linear range of the calibration graph was 0-5 00 ng/mL, The limit of detection(3 sigma) was 1.54 ng/mL, A new method of r esonant luminescence for the determination of trace DNA was established wit h high sensitivity and selectivity.